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# Slicer is free software: you can redistribute it and/or modify
# it under the terms of the GNU General Public License as published by
# the Free Software Foundation, either version 3 of the License, or
# (at your option) any later version.
# Slicer is distributed in the hope that it will be useful,
# but WITHOUT ANY WARRANTY; without even the implied warranty of
# GNU General Public License for more details.
# You should have received a copy of the GNU General Public License
# along with Slicer. If not, see .
# – Try to find CImg-2.2
# Once done, this will define
# CImg_FOUND – system has CImg-2.2
# CImg_INCLUDE_DIRS – the CImg include directories
# CImg_LIBRARIES – link these to use CImg

find_package( CImg 2.2 REQUIRED )
include_directories( ${CImg_INCLUDE_DIRS} )

message(FATAL_ERROR « CImg required (missing: ${CImg_LIBRARIES}) »)

# define the imported targets and variables
add_library( CImg SHARED IMPORTED )

# Set additional Library_Imported_Targets (Imported from CImg)


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It is not currently known which of the recently introduced human-specific miRNAs play a role during the infectious life cycle in ticks, or if and how they are involved in the regulation of viral replication, viral transmission and the establishment of infection. Moreover, most recent studies target a specific miRNA, the expression of which is known to be strongly affected by viral infection. As these methods are highly sensitive, the detection of v-miRNAs may lead to different conclusions from those obtained by other authors using lower sensitivity techniques ([@B31],[@B49],[@B50]).

Finally, the study of viral miRNAs expression in different developmental stages and tissues has shown that they are expressed in a stage-dependent and tissue-specific manner. For example, viral miRNAs have been found to be differentially expressed in the SG of unfed, fed and blood-engorged female ticks ([@B28]), in salivary glands of rabbits infected by tick-borne encephalitis virus ([@B51],[@B52]) and in pools of eggs, larvae and newly fed nymphs of the Simulium blackfly vector of the Omsk hemorrhagic fever virus ([@B53]).

Overall, high-throughput sequencing techniques provide a powerful tool for the identification of new viral miRNAs, and enable new insights to be obtained into the complex regulatory networks involved in the infectious cycle of different viruses. Identification of viral miRNAs and understanding their roles in the pathogen-host relationship may lead to the identification of new molecular targets for the development of novel antiviral strategies.


This work was supported by grants from the Generalitat de Catalunya (2009 SGR-709). M.I.S. is a recipient of a Ramón y Cajal contract from the Spanish Ministry of Education and Science (RYC-2011-07692).

*Conflict of interest statement*. None declared.

[^1]: ^a^denoted by 7B, 7C and 7D v-miRNAs are derivatives of the 7B-1 v-miRNA precursor and display the same sequence, but the 7B-1 7D mature miRNA is derived from the 7B-1–1 position and the 7D is derived from the 7D-2 position of the precursor.




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